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Concentration and purification of lycopene from watermelon juice by integrated microfiltration-based processes

机译:通过基于微滤的集成工艺从西瓜汁中浓缩和纯化番茄红素

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摘要

The concentration of thermosensitive bioactive compounds remains an important challenge. A gentle integrated process including enzymatic maceration, crossflow microfiltration, diafiltration and centrifugation was applied to watermelon juice to obtain a natural extract enriched with lycopene. Microfiltration was performed using ceramic membranes with different pore diameters (0.2 to 1.4 μm), transmembrane pressures (50 to 200 kPa) and temperatures (50 °C and 60 °C). Using the best operating conditions during microfiltration, the permeate flux was close to 110 L·h− 1·m− 2, and the lycopene concentration increased 11-fold in the retentate. Diafiltration allowed for the purification of lycopene up to 25-fold. Centrifugation enhanced the lycopene concentration up to 4-fold. cis-Isomerization remained low and similar to that of raw juice. This integrated process obtained a natural extract of lycopene that was 41 times more concentrated and 34 times purer than the initial juice, yielding an extract with up to 2% all-trans-lycopene on a dry basis. (Résumé d'auteur)
机译:热敏生物活性化合物的浓度仍然是重要的挑战。对西瓜汁采用包括酶浸渍,错流微滤,渗滤和离心的温和的综合方法,以获得富含番茄红素的天然提取物。使用具有不同孔径(0.2至1.4μm),跨膜压力(50至200 kPa)和温度(50°C和60°C)的陶瓷膜进行微滤。在微滤过程中使用最佳操作条件,渗透通量接近110 L·h-1·m-2,番茄红素的浓度在渗余物中增加了11倍。渗滤允许将番茄红素纯化多达25倍。离心将番茄红素的浓度提高了4倍。顺式异构化仍然很低,与原汁相似。这种综合方法获得了番茄红素的天然提取物,其浓缩度是原始汁液的41倍,纯度是原始汁液的34倍,在干基中生成的提取物含高达2%的全反番茄红素。 (Résuméd'auteur)

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